Principal Investigator
Prof. Ing. Stanislav Kmoch, CSc. (skmoch@lf1.cuni.cz; 224967691)
Research interest
is a professor of medical biology and genetics at First Faculty of Medicine, of Charles University in Prague. There he leads a Research Unit for Rare Diseases which is associated with the Department of Pediatrics and Adolescent Medicine. He is also appointed as Adjunct professor at the Dept. of Internal Medicine, Section of Nephrology, Wake Forest School of Medicine, North Carolina, USA and visiting scientist at the Broad Institute of MIT and Harvard, Boston, USA.
He is mostly interested in elucidation of the genetic and molecular basis of rare genetic diseases and characterization of the role of rare genetic variants in complex diseases. He has contributed to more than 100 research papers that have been published in prestigious high impact journals such as Cell, New England Journal of Medicine, Nature Genetics, Nature Communications, Journal of Clinical Investigation, American Journal of Human Genetics, Cell Metabolism, Human Molecular Genetics, Human Mutation, Nature Neuroscience (cumulative IF 670; 3000 citations, H-index 31) and his work was awarded by several national awards.
Publications
Link to pubmed papers https://www.ncbi.nlm.nih.gov/pubmed/?term=Kmoch+S
Senior researchers
Pavel Martásek
Prof. Jiri Zeman, MD, PhD, ( Jiri.Zeman@lf1.cuni.cz )
Prof. Jiri Zeman, MD, PhD, professor of paediatrics at the Department of Paediatrics, First Faculty of Medicine, Charles University, Prague, is involved in the research projects focused on inherited metabolic disorders on the clinical, biochemical and molecular levels. These projects significantly contributed to characterization of several novel genes and proteins and their role in men in pathological conditions.
Together with his PostDocs and PhD students he contributed to 110 research papers published in impact journals including Nature Genetics, Lancet, American Journal of Human Genetics and Brain. The published results have 3000 citations and his H-index is 32. His work was awarded by several national awards. He is also engaged in the pre- and postgradual teaching programs for students and paediatricians. The Department of Paediatrics was under his supervision accredited by EAP-U.E.M.S. as the European Training Centre in Metabolic Diseases.
Publications
Link to pubmed papers https://www.ncbi.nlm.nih.gov/pubmed/?term=Zeman + J
doc. MUDr. Petra Lišková, M.D., Ph.D. , (Petra.Liskova@lf1.cuni.cz)
Associate Professor in Medical Genetics and Biology, Charles University, Prague where is act as a group leader. In addition, she has founded the only specialized clinic for patients with inherited ocular disorders within the Czech Republic. She is also appointed as Honorary Research Associate at the UCL Institute of Ophthalmology, UK and Visiting Professor at the School of Optometry and Vision Sciences, Cardiff University, UK
Her main research interest lies in the genetics of complex and monogenic eye diseases. Her key findings include identification of the disease-causing gene for posterior polymorphous corneal dystrophy type 1 (OVOL2) and type 4 (GRHL2). She has contributed to more than 80 research papers that have been published in prestigious high impact journals such as American Journal of Human Genetics, Lancet Neurology, Human Mutation, European Journal of Human Genetics (>1200 citations, H-index 19)
Link to published papers https://orcid.org/0000-0001-7834-8486
Post-docs (junior researchers)
Ing. Ľubica Ďuďáková, PhD. (lubica.dudakova@lf1.cuni.cz; 224967139)
Research interest
is a research fellow at First Faculty of Medicine, of Charles University in Prague. She has contributed to almost 40 research papers that have been published in prestigious high impact journals such as American Journal of Human Genetics, European Journal of Human Genetics and Investigative Ophthalmology and Visual Science (280 citations, H-index 8).
Her main interest is in the genetics of complex and monogenic eye diseases. Her key findings include identification of the disease-causing gene for posterior polymorphous corneal dystrophy type 1 (OVOL2) and type 4 (GRHL2), finding that ZEB1 haploinsufficiency is one the disease mechanisms in posterior polymorphous corneal dystrophy type 3 and elucidating the role of expansion in TCF4 gene in the pathogenesis of common age-related condition Fuchs corneal endothelial dystrophy. She refines ocular phenotypes of a number of rare monogenic corneal disorders (Harboyan syndrome, cornea plana, macular corneal dystrophy, posterior polymorphous corneal dystrophy).
Publications
Link to pubmed papers https://www.ncbi.nlm.nih.gov/pubmed/?term=dudakova+L
Viktor Stránecký, Ph.D. (viktor.stranecky@lf1.cuni.cz ; 224967668 )
Research interest
is a post-doc research scientist at the Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University in Prague.
His main research interest lies in the bionformatics and in elucidation of molecular basis of rare genetic diseases by new sequencing technologies. His key findings include identification of the disease-causing gene for ATP synthase deficiency (TMEM70), neuronal ceroid lipofuscinosis type 4 (DNAJC5), Rotor syndrome (OATP1B1 and OATP1B3), posterior polymorphous corneal dystrophy 1 (OVOL2),and Gapo syndrome (ANTXR1) . He has contributed to 56 research papers that have been published in prestigious high impact journals such as American Journal of Human Genetics, Cancer Cell, Nature Communications, Journal of Clinical Investigation, Nature Genetics, Nature Neuroscience (>1050 citations).
Publications
Link to pubmed papers https://www.ncbi.nlm.nih.gov/pubmed/?term=Stranecky+V
RNDr. Hana Hartmannová, Ph.D. (hana.hartmannova@lf1.cuni.cz ; 224967052)
Research interest
She is a post-doc junior researcher and a member of Research Unit for Rare Diseases at the Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University in Prague.
She is interested in elucidation of molecular basis of rare genetic diseases by new sequencing technologies. She contributed to the identification of disease causing genes of adenylosuccinate lyase deficiency (ADSL), MPSIIIC (TMEM76) and ATP synthase deficiency (TMEM70), Rotor syndrome (OATP1B1 and OATP1B3), adult neuronal ceroid lipofuscinosis (DNAJC5), GAPO syndrome (ANTXR1), X-linked hypertrophic cardiomyopathy (FHL1), protein glycosylation deficiency (Nogo-b receptor), Oliver-McFarlane syndrome (PNPLA6), posterior polymorphous corneal dystrophy (OVOL2) and renal Fanconi syndrome (NDUFAF6).
She has contributed to 41 research papers published in prestigious high impact journals such as Nature Genetics, Human Molecular Genetics, Journal of Clinical Investigation or American Journal of Human Genetics (H-index 16, 863 times cited).
Publications
Link to pubmed papers https://orcid.org/0000-0001-7787-832X
RNDr. Ladislav Kuchař Ph.D. (Ladislav.Kuchar@lf1.cuni.cz )
Research interest
is post-doc junior researcher of medical biology and biochemistry at First Faculty of Medicine, of Charles University in Prague. There he is a member of Research Unit for Rare Diseases which is associated with the Department of Pediatrics and Adolescent Medicine.
He is mostly interested in pathobiochemistry and lipidomic mass spectrometry application in research and diagnostic of rare diseases with particular focus on lysosomal storage disorders. He also cooperates with other research groups in field of early cancer diagnostic and sphingolipids role in cell biology. He has contributed to 14 research papers that have been published in journals such as Nature Communications (cumulative IF 63; 120 citations, H-index 7).
Publications
Link to pubmed papers https://www.ncbi.nlm.nih.gov/pubmed/?term=Kuchar+L
Ing. Dita Mušálková, PhD (dita.musalkova@lf1.cuni.cz; 224967211)
Research interest
Dita Mušálková is a junior researcher at the Research Unit for Rare Diseases, Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University in Prague. She is a graduate of the Institute of Chemical Technology in Prague (Clinical Bioanalytics) and completed her PhD training in Molecular and cell biology, Genetics and Virology at the First Faculty of Medicine, Charles University. She has contributed to 9 research papers published in journals, such as Rheumatology, Gene, and Clinica Chimica Acta (H-index 3). She is particularly interested in X-linked diseases and her key results include the development of novel methods for the measurement of X-inactivation ratios and application of these assays in X-linked metabolic disorders, e.g. mucopolysaccharidosis type II, OTC and HPRT deficiency, and PRPS1 superactivity. Her other interests lie in the field of lysosomal storage disorders, where she worked on the description of the promoter of HGSNAT gene encoding the lysosomal enzyme defective in mucopolysaccharidosis type IIIC and characterization of pathogenic processes in NPC1-deficient cells. This work lead to a patent application of a novel test for diagnostics of Niemann-Pick disease type C and other defects of cholesterol metabolism. Lately, she has focused on processing and evaluation of the data generated by new sequencing technologies.
Publications
Link to pubmed papers https://orcid.org/0000-0003-4795-3960
Mgr. Lenka Nosková, Ph.D. (lnosk@lf1.cuni.cz ; 224967169)
Research interest
She is a post-doc junior researcher and a member of Research Unit for Rare Diseases at the Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University in Prague.
She is interested in elucidation of molecular basis of rare genetic diseases by new sequencing technologies. She contributed to the identification of disease causing genes of mucopolysaccharidosis type IIIC (TMEM76), ATP synthase deficiency (TMEM70), neuronal ceroid lipofuscinosis type 4 (DNAJC5), Rotor syndrome (OATP1B1 and OATP1B3), posterior polymorphous corneal dystrophy 1 (OVOL2), Gapo syndrome (ANTXR1) and X-linked hypertrophic cardiomyopathy (FHL1).
She has contributed to 19 research papers published in prestigious high impact journals such as Nature Genetics, Journal of Clinical Investigation or American Journal of Human Genetics (cumulative IF 125, H-index 12).
Publications
Link to pubmed papers https://orcid.org/0000-0001-7011-4327
RNDr. Alena Vrbacká, Ph.D. (maiden name: Čížková)
(acizk@lf1.cuni.cz; 224 967 680)
Research interest
She is a post-doc junior researcher and a member of Research Unit for Rare Diseases at the Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University in Prague.
She finished her Ph.D. training in Biomedicine, section Biochemistry and Pathobiochemistry, First Faculty of Medicine, Charles University, Prague in 2009.
She focuses her research on the understanding of renal pathophysiology, especially the genes of uromodulin (UMOD) and mucin (MUC1). She is also interested in elucidation of molecular basis of rare genetic diseases by new sequencing technologies. She contributed to the identification of disease causing genes of mucopolysaccharidosis type IIIC (TMEM76) and ATP synthase deficiency (TMEM70).
She has contributed to 8 research papers published in prestigious high impact journals such as Nature Genetics, The American Journal of Human Genetics, Journal of American Society of Nephrology (H-index 7, 310 times cited).
Publications:
http://www.researcherid.com/rid/F-4100-2011
ORCID 0000-0003-4640-1646
Mgr. Martina Živná, Ph.D. (maiden name: Martina Kublová)
mzivna@LF1.cuni.cz ; 224967668)
Research interest
she finished her Ph.D. training in Molecular biology, Genetics and Virology at the Institute of Inherited Metabolic Diseases (First Faculty of Medicine – Charles University, Prague, Czech Republic) in 2010.
Dr. Zivna is a senior scientist in clinical-research team led by prof. Stan Kmoch at the Department of Pediatrics and Adolescent Medicine (First Faculty of Medicine – Charles University).
She focuses her research efforts at understanding the etiology and pathogenic cascades involved in progression of inherited chronic kidney diseases. She previously identified variants in renin (REN) and translocon subunit SEC61A (SEC61A1) genes as genetic cause of autosomal dominant tubulointerstitial kidney disease (ADTKD) and contributed to functional characterization of mutations in uromodulin (UMOD) and mucin-1 (MUC1). She also recently developed a noninvasive urine-based immunolabeling technique for diagnosis of ADTKD caused by mutation(s) in MUC1.
She has contributed to 19 research papers that have been published in prestigious high impact journals such as Cell, American Journal of Human Genetics, Journal of American Society of Nephrology, Kidney International, Human Molecular Genetics.
Last, Dr. Zivna curates a multi-language (Czech and Russian) web platform (http://selhaniledvin.cz/) that provides counseling and networking support to patients (and their nephrologists) with inherited chronic kidney diseases.
Publications
Link to pubmed papers https://www.ncbi.nlm.nih.gov/pubmed/?term=Zivna%20m%20or%20Kublova%20m
Hana Kolarova, M.D., Ph.D. (hana.kolarova@vfn.cz, 224 967 791)
Dr. Kolarova graduated in General Medicine from the 1st Medical Faculty at Charles University in Prague in 2013. She did her residency in Department of Paediatrics, General University Hospital and got a certificate in paediatrics in 2018. She has currently been working as an attending physician and medical students' teacher at a Department for Sick Neonates and Infants, Metabolic Ward and Metabolic Outpatient Clinic.
Her major research interest concerns inherited metabolic disorders, in particular mitochondrial diseases. During her doctoral studies in Biochemistry and Pathobiochemistry she focused on the characterization of mitochondrial biogenesis within foetal development. Her current research interest in Laboratory for Study of Mitochondrial Diseases aims at clinical, biochemical and molecular aspects of mitochondrial optic neuropathies. With this project, she was awarded several student prizes and received important research fundings. She is cooperating with other european reference centres, especially Friedrich-Baur-Institut at Ludwig-Maxmilians-University in Munich and Institut Imagine by Necker Hôpital Necker-Enfants malades at Université Paris Descartes-Sorbonne in Paris, where she was on educational stays. She has contributed to over 15 scientific papers (cummulative IF 34) and two monographs.
Kytnarová
RNDr. Nina Ondrušková, Ph.D. (nina.ondruskova@lf1.cuni.cz; 224967748)
Research interest
is a junior researcher at the Laboratory for Study of Mitochondrial Disorders, Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine of Charles University in Prague. After finishing her PhD studies on the congenital disorders of glycosylation (CDG) in 2016, she conducted a 6-month postdoc stay in the laboratory of Daan Van Aalten (University of Dundee, Dundee, Scotland) focused on O-GlcNAc research.
She contributed to the biochemical and molecular characterization of various CDG types such as RFT1-CDG, MAN1B1-CDG, PGM1-CDG, NGBR-CDG, SLC10A7-CDG or ATP6AP1-CDG, and the results of her scientific work have been published in a number of impacted journals including J Inherit Metab Dis, Mol Genet Metab, Brain, Cell Metab, Metabolism or Hum Mol Genet (12 publications; 118 citations; H-index 6).
Publications
Link to pubmed papers:
https://www.ncbi.nlm.nih.gov/pubmed/?term=Ondruskova+N
Tesařová
Mgr. Iveta Tóthová, Ph.D. (Iveta.Tothova@lf1.cuni.cz)
Research interest
Is a junior researcher at the Laboratory for Study of Mitochondrial Disorders, Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine of Charles University in Prague. Her main interest is genetic basis of mitochondrial diseases and other rare diseases.
During her Master and Ph.D. studies, to expand her knowledge in genetic research she took a part in a:
- three months Erasmus internship at the Department of Immunology, Genetics and Pathology at Uppsala University in Sweden
- two months internship in Department of Molecular and Cellular Biology at the University of Guelph in Canada
- one and two months internship in Institute of Cardiovascular Science at University College London in United Kingdom
She has contributed four research papers that have been published in European Journal of Human Genetics, Journal of Applied Genetics and Journal of Clinical Laboratory Analysis (19 citation; H-index 2).
Publications
Link to pubmed papers: https://www.ncbi.nlm.nih.gov/pubmed?term=(Tothova%2C%20Iveta%5BAuthor%5D)%20AND%20University%20of%20Presov%5BAffiliation%5D
MUDr. Jana Tukova PhD. jana.tukova@lf1.cuni.cz (tel.:224967743)
graduated in general medicine at the First Medical Faculty of Charles University in Prague. Since then she has worked as a resident and PhD student at the Department of Paediatrics at General University Hospital in Prague. She completed her doctoral research project dealing with the pharmacokinetics of methotrexate used in children with juvenile idiopathic arthritis. After graduation in Paediatrics, she has started training in Paediatric Pulmonology. She coordinated respiratory working subgroup in project New methods in follow-up of children with perinatal burden at Centre for Follow up Care of Ex- Preterm Children with focus on bronchopulmonary dysplasia and its long-term consequences and she led project ImmaturCare aimed at prediction of chronic morbidity in ex-preterm children as chief coordinator. At the beginning of 2019, she completed extensive Textbook of Paediatric Pulmonology with two other editors. Currently, she concentrates her research on chronic lung disease of immaturity, its prediction and possible prevention.
Link to pubmed papers:
https://orcid.org/0000-0001-6655-1073
MUDr. Pavla Pokorná, PhD (224967779; 224911453)
is a neonatologist and pediatrician interested in clinical pharmacology in neonates and children at the First Faculty of Medicine, of Charles University in Prague and General University Hospital.
There she builts as a member and one of co-founders of a networking group (CzechPharmNet) based on local and international collaboration (the international European consortium called c4c, Connect4Children - Collaborative network for European clinical trials for children) clinical pharmacology in neonates and children.It is associated with the department of Pediatrics and Adolescent Medicine of the First Faculty of Medicine and the General University Hospital in Prague the Institute of Pharmacology and the Institute of Pharmacology of the First Faculty of Medicine of Charles University. She is also appointed as postgraduate student at the Intensive Care and Department of Pediatric Surgery, Erasmus MC - Sophia Childrens Hospital, Rotterdam, the Netherlands The development of pediatric pharmacology takes place at several levels: 1. undergraduate education, 2. postgraduate education, 3. routine pediatric practice (inpatient and outpatient), and 4. research. Pharmacology research is based on a systematic approach of postgraduate studies, interdisciplinary cooperation with other disciplines beyond the scope of pediatrics and neonatology, international cooperation with different institutions (Erasmus Rotterdam University – prof. Dick Tibboel, prof. John van den Anker, prof Karel Allegaert), Karolinska Institut – prof per Arne Lonnquist), experts in the field - prof. Karel Allegaert (University of Leuven), Dr. Birgit Koch Easmus MC), prof. Catherijne Knibbe, Elke Krekels (University of Leiden), prof. John van den Anker (University of Basel), prof Tom Giedsing Hansen (Odense Universitaethospital). She is active in international professional societies – co-chair of the section Pharmacology of the European Society of Paediatric Neonatal Intensive Care -ESPNIC). She defended the thesis on clinical pharmacology at the first Faculty of Medicine, of Charles University of Prague in Hradec Králově 2014.
She has contributed to more than 39 research papers that have been published in impact journals (cumulative IF 32; 241 citations, H-index 8)
Publications
Link to pubmed papers https://www.ncbi.nlm.nih.gov/pubmed/?term=Pokorna.P
Farrag
Ing. Milan Jakubek, Ph.D. milan.jaboubek@lf1.cuni.cz
Milan Jakubek is a graduate of the Institute of Chemical Technology in Prague, Faculty of FCHI, where he also successfully defended his Ph.D.
During his studies and subsequently he devoted himself to basic and applied research both within R&D institutions and in the field of industrial pharmacy and chemistry in the Czech Republic, Europe and the world. He has extended his experience with internships in the European Parliament and the Swiss Institute of Technology FHNW.
Currently he is a lecturer at the First Faculty of Medicine of Charles University in the Center of Excellence BIOCEV. Specialization in the fields of medical chemistry, analytical chemistry, drug development, formulation or teranostic. Its activities are focused on basic and applied research and innovation. The scientific experience is based on the management of basic and applied research and technology transfer into practice. This experience is supported by authorship and co-authorship of impacted publications, used national and international patents and cooperation with partners from the medical, chemical and pharmaceutical industries.
He has contributed to 17 research papers that have been published, 1x US and 1x EU patent, 3x CZ patent, 7x utility models (133 citations, H-index 7).
Link to pubmed papershttps://www.researchgate.net/profile/Milan_Jakubek/research
Malina
Tatar
Other team members
Petr Vyleťal, Ph.D. (pvyle@lf1.cuni.cz)
is a post-doctoral research scientist at First Faculty of Medicine of Charles University in Prague in the group of Prof. Stanislav Kmoch at Research Unit for Rare Diseases. By training, Petr is biomedical engineer (ICT Prague, 2001) and obtained his Ph.D. at First Faculty of Medicine in Molecular and Cell Biology, Genetics and Virology study program (2009). He is also Registered Bioanalyst for Clinical Genetics (IPVZ Prague, 2011).
Research interest
Petr works as biochemist, molecular biologist and geneticist in the field of rare autosomal dominant kidney diseases (ADTKD). As a main author or co-author, he contributed to elucidation of molecular basis of this group of disorders (16 publications, 469 citations, H-index 11 according to Publons). He participates in collaborations with domestic (General Faculty Hospital in Prague) as well as foreign scientific institutions (Broad Institute, Wake Forest School of Medicine).
Publications: https://orcid.org/0000-0002-9357-1237
RNDr. Hana Hansíková, CSc.(hana.hansikova@lf1.cuni.cz; 224967748)
She is head of Laboratory for Study of Mitochondrial Disorders (MITOLAB) at Department of Pediatrics, Charles University and General University Hospital in Prague. She has worked as a biochemical expert in the field of metabolic disorders, especially in bioenergetics of mitochondrial disorders and other various pathological conditions for more than 25 years. She contributed to more than 100 research papers that have been published in different impact journals such as Nature genetics, Scientific Reports, Metabolism, Disease model and mechanisms , Human Molecular Genetics, Brain , Cell Metabolism, Mitochondrion, Oncotarget, Biochemical Journal, International Journal of Obesity , Journal of Medical Genetics, PloS One, Journal of inherited metabolic disorders, Translational Research, Neurodegenerative Diseases, Current Alzheimer Research (H-index 22). Topic research areas: 1. Functional, structural and biochemical characterization of various primary mitochondrial diseases; 2. Maturation of mitochondrial energy metabolism; 3. Congenital disorders of glycosylation; 4. Mitochondrial metabolism in neurodegeneration.
Publications:
Link to pubmed papers: https://www.ncbi.nlm.nih.gov/pubmed/?term=Hansikova+H
Doc. MUDr. Tomáš Honzík, Ph.D. (tomas.honzik@lf1.cuni.cz, 224967733)
Research interest
Dr. Honzík is an associate professor of pediatrics at First Faculty of Medicine at Charles University in Prague. He is the head of the Department of Pediatrics and Adolescent Medicine. Dr. Honzík has a long-standing clinical expertise in pediatrics and clinical genetics. He is involved in the diagnostics and management of inherited metabolic diseases since 2000. His research is focused on mitochondrial disorders and congenital disorders of glycosylation.
Most significant publications: Clinical characterization and identification of TMEM70 as a novel factor of ATPase biogenesis (Nat Genet, 2008; Arch Dis Child, 2010; J Inherit Metab Dis, 2015), NgBR deficiency as a novel subtype of congenital disorders of glycosylation caused by dolichol synthase defect (Cell Metab, 2014), DCDC2 deficiency as a cause of renal-hepatic ciliopathy (Am J Hum Genet, 2015), ITPA deficiency leading to infantile encephalopathy (Ann Neurol, 2015), Vps15 deficiency which perturb neuronal migration in mice and is associated with neurodevelopmental disease in humans caused by impairment of phospholipid metabolism influencing endosomal-lysosomal trafficking and autophagy (Nat Neurosci, 2018) and uncovering an important role of SLC10A7 protein in bone mineralization and transport of glycoproteins to the extracellular matrix (Hum Mol Genet, 2018).
In total, he (co-)authored more than 100 peer reviewed papers on inherited metabolic diseases, clinical genetics and pediatrics (cumulative IF 279; 976 citations, H-index 18).
Publications
Link to pubmed papers https://www-ncbi-nlm-nih-gov.ezproxy.is.cuni.cz/pubmed/?term=Honzik+T
Králík
RNDr. Jarmila Králová, CSc. (jarmila.kralova@lf1.cuni.cz, kralova@img.cas.cz; 241063392)
She is senior researcher at Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University in Prague and at the Institute of Molecular Genetics Czech Academy of Sciences in Prague.
She is at presently interested in the development and characterization of fluorescent probes for targeting biologically important molecules and cellular compartments. Her long-term interests are also oncogenic transformation, induction of apoptosis by macrocyclic compounds, photodynamic therapy, and targeted drug delivery.
Publication activity: She is the author or co-author of 2 book chapters, 16 patents and 75 articles published in international peer-reviewed journals (WOS: 1737 citations, H-index 25)
Link to pubmed papers:
https://orcid.org/0000-0002-4735-8857
Tlhakanelo